【文献标题】Emerging contaminant 1,3,6,8-tetrabromocarbazole induces oxidative damage and apoptosis during the embryonic development of zebrafish (Danio rerio)

【作者】Jingwen Zhang，Cheng Zhang，Zhongkun Du，et.al

【作者单位】山东农业大学（Shandong Agricultural University）

【文献中引用产品】

斑马鱼8羟基脱氧鸟苷（8-OHdG）ELISA试剂盒

斑马鱼抑肽酶（AP）ELISA试剂盒

斑马鱼总蛋白（TP）ELISA试剂盒

斑马鱼脂质过氧化物酶（LPO）ELISA试剂盒

抗氧化酶（AOE）ELISA试剂盒

【关键词】Polyhalogenated carbazoles，Zebrafish，Developmental toxicity，Antioxidant enzymes，Apoptosis

【DOI】doi.org/10.1016/j.scitotenv.2020.140753

【影响因子(IF)】5.92

【出版期刊】《Science of the Total Environment》

【产品原文引用】

Chemicals

1368-TBCZ (C12H5Br4N, CAS 55119-09-0, purity N95%) was purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). Dimethyl sulfoxide (DMSO, 99.9% pure, Sigma, Missouri, USA) was used to prepare stock solutions. Kaitong Chemical Reagent Co., Ltd. (Tianjin,China) and Solarbio Science & Technology Co. (Beijing, China) provided the other chemicals (analytical grade). We used kits from Nanjing Jiancheng Bioengineering Research Institute (Jiangsu), Hengyuan Biotechnology Co., Ltd. (Shanghai), Solar Technology Co., Ltd. (Beijing,China) to measure total protein and lipid peroxidation levels, three measures of antioxidant activity changes, 8-OHdG content level, and apoptosis rate. The primers, reagents, and kits for gene expression measurement were purchased from CoWin Biosciences Co., Ltd. (Beijing,China) and Sangon Biotechnology Co., Ltd. (Shanghai, China)

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