【文献标题】Roles of antioxidant capacity and energy metabolism in the maturitydependent chilling tolerance of postharvest kiwifruit

【作者】Feng Wanga，Qingzhen Yanga，Qifeng Zhao，et.al

【作者单位】运城学院（Yuncheng University）

【文献中引用产品】

试剂-蔗糖

【关键词】Kiwifruit，Maturity，Chilling injury，Antioxidant capacity，Energy metabolism

【DOI】doi.org/10.1016/j.postharvbio.2020.111281

【影响因子(IF)】4.43

【出版期刊】《Postharvest Biology and Technology》

【产品原文引用】

Energy metabolism enzyme activity measurement

bath and centrifuged at 4000 g for 15 min at 4 ℃. The supernatant was rapidly extracted and centrifuged at 15,000 g for 20 min at 4 ℃,and the resulting precipitate was washed in 10 ml 10 mmol L-¹ Tris−HCl (pH 7.5) (Sigma Aldrich, Missouri, USA), which contained 0.3 mol L-¹ mannitol (Herochem Co. Ltd., Shanghai, China), 1.0 mmol L-¹ EDTA (Tokyo Chemical Industry, Tokyo, Japan), and 0.25 mol L-¹ sucrose (Hengyuan Biochemical Reagent Co. Ltd., Shanghai, China), in an ice-water bath. The wash buffer was centrifuged at 4000 g for 10 min at 4 °C, and the supernatant was centrifuged at 15,000 g for 20 min at 4 ℃. The final precipitate, which was made up of mitochondria, was then eluted in 1.5 ml 10 mmol L−¹ Tris−HCl (pH 7.5), which contained 0.3 mol L-¹ mannitol (Herochem Co. Ltd., Shanghai, China), 1.0 mmol L-1 EDTA (Tokyo Chemical Industry, Tokyo, Japan), and 0.25 mol L-¹ sucrose (Hengyuan Biochemical Reagent Co. Ltd., Shanghai, China),and used for the following analyses.

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