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技术文献:小鼠8羟基脱氧鸟苷,小鼠过氧化脂质ELISA试剂盒引用文献
更新时间:2021-01-08      阅读:789

【文献标题】Reparative effects of lycium barbarum polysaccharide on mouse ovarian injuries induced by repeated superovulation

【作者】Bing Liu,Jun-Li Wang,Xin-Mei Wang,et.al

【作者单位】北京农业大学(Beijing University of Agriculture)

【文献中引用产品】

小鼠8羟基脱氧鸟苷(8-OHdG)ELISA试剂盒

小鼠过氧化脂质;乳过氧化物酶(LPO)ELISA试剂盒

【关键词】Superovulatory times,Injury model,LBP,Remedy,Oxidative stress

【DOI】doi.org/10.1016/j.theriogenology.2020.01.048

【影响因子(IF)】2.33

出版期刊】《Theriogenology》

【产品原文引用】

8-OHdG and LPO levels of the collected ovarian tissues were determined using an ELISA kit, according to the manu-facturer’s instructions (Shanghai Hengyuan Biotechnology Co., Ltd,Shanghai, China). The collected mouse ovaries were cracked with lysate. Each 10 mL henylmethane-sulfonyl (PMSF) was added in 1 mL radio immuno precipitation assay (RIPA) as lysate. Every 1 g of tissue with 9 mL lysate, then coated with mouse antibody against 8-OHdG or LPO. After incubation and washing concentrations of 8-OHdG or LPO were determined as described previously .Intra-and inter-CVs: 8-OHdG (6.3%, 8.1%), LPO (5.2%, 7.4%).

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