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详情介绍
恒远生物提供免费代测服务,产品仅供科研使用。
产品名称:人胰岛素样生长因子结合蛋白4(IGFBP-4 )
英文名称:Human insulin-like growth factors binding protein 4,IGFBP-4 ELISA KIT
产品规格:96人份/48人份
主要组成成分:试剂盒
性状:液体
适应症:科研
贮藏:2-8℃
人胰岛素样生长因子结合蛋白4(IGFBP-4 )计算
以标准物的浓度为横坐标,OD值为纵坐标,在坐标纸上绘出标准曲线,根据样品的OD值由标准曲线查出相应的浓度;再乘以稀释倍数;或用标准物的浓度与OD值计算出标准曲线的直线回归方程式,将样品的OD值代入方程式,计算出样品浓度,再乘以稀释倍数,即为样品的实际浓度。
Steps description(英文)
Standard, Sample diluent |
Add Standard, Sample diluent, incubate for 30 min at |
Wash 5 time,Add HRP-Conjugate reagent, incubate for 30 min at |
Wash 5 times,Add Chromogen Solution A and B, incubate for 30 min at |
Add Stopp Solution |
Read absorbance at 450nm within 15 min |
calculate |
Calculate
Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density and the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.
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