产品名称：人抗利尿激素（ADH）

英文名称：  Human antidiuretic hormone/vasopressin/arginine vasopressin,ADH/VP/AVP ELISA KIT                                                             

产品规格：96T/48T                                                                      

品牌：RD/RB                                                                  

类别：ELISA试剂盒/人的ELISA检测试剂盒                                             

上海恒远生物公司全部备有现货，有需要的请及时垂询：,

“ 人抗利尿激素（ADH）”操作步骤（中文）                                                                 

1.标准品的稀释：本试剂盒提供原倍标准品一支，用户可按照下列图表在小试管中进行稀释。                                                                   

8 ng/L    5号标准品  150μl的原倍标准品加入150μl标准品稀释液                                                   

4 ng/L    4号标准品  150μl的5号标准品加入150μl标准品稀释液                                                   

2 ng/L    3号标准品  150μl的4号标准品加入150μl标准品稀释液                                                   

1 ng/L    2号标准品  150μl的3号标准品加入150μl标准品稀释液                                                   

0.5 ng/L 1号标准品  150μl的2号标准品加入150μl标准品稀释液                              

2.加样：分别设空白孔（空白对照孔不加样品及酶标试剂，其余各步操作相同）、标准孔、                                             

待测样品孔。在酶标包被板上标准品准确加样50μl，待测样品孔中先加样品稀释液40μl，然后                                                

再加待测样品10μl（样品zui终稀释度为5倍）。加样将样品加于酶标板孔底部，尽量不触                                              

及孔壁，轻轻晃动混匀。                                              

3.温育：用封板膜封板后置37℃温育30分钟。                                               

4.配液：将20倍浓缩洗涤液用蒸馏水20倍稀释后备用                              

5.洗涤：小心揭掉封板膜，弃去液体，甩干，每孔加满洗涤液，静置30秒后弃去，如此重复                                                

5次，拍干。                                              

6. 加酶：每孔加入酶标试剂50μl，空白孔除外。                                              

7.温育：操作同3。                                          

8.洗涤：操作同5。                                          

9.显色：每孔先加入显色剂A50μl，再加入显色剂B50μl，轻轻震荡混匀，37℃避光显色15分钟.                                            

10.终止：每孔加终止液50μl，终止反应（此时蓝色立转黄色）。                   

11.测定：以空白空调零，450nm波长依序测量各孔的吸光度（OD值）。 测定应在加终止液后                                             

15分钟以内进行。                                           

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上海恒远生物公司长期供应进口“ ”，试剂品质可靠，价格从优，有意者请,